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Rabbit anti-NAT10 Antibody
SKU: orb1523089
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 675 and 725 |
| Target | NAT10 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−Kre33; RNA cytidine acetyltransferase; NET43; N-acetyltransferase-like protein; N-acetyltransferase 10 (GCN5-related); ALP; 18S rRNA cytosine acetyltransferase; N-acetyltransferase 10
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−- Item 1 of 4
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Detection of human NAT10 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody orb1523089 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human NAT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody orb1523089 used for IP at 6 µg per reaction.
Detection of human NAT10 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NAT10 (Cat. No. orb1523089) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of mouse NAT10 by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-NAT10 (Cat. No. orb1523089) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_078938.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-NAT10 Antibody (orb1523089)
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