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Rabbit anti-NEDD-9/CASL/HEF1 Recombinant Monoclonal Antibody
SKU: orb1733239
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB: 1:1000, IP: 6 µl/mg lysate |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Clone No. | BLR248L |
| Immunogen | Between 150 and 200 |
| Target | NEDD-9/CASL/HEF1 |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−CRK-associated substrate-related protein; Cas scaffolding protein family member 2; p130Cas-related protein; p105; neural precursor cell expressed developmentally down-regulated protein 9; NEDD-9; HEF1; enhancer of filamentation 1; renal carcinoma antigen NY-REN-12; Crk-associated substrate related protein Cas-L; CASS2; cas-like docking; CAS-L; CASL; CAS2; Enhancer of filamentation 1 p55
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−- Item 1 of 3
Rabbit anti-NEDD-9/CASL/HEF1 Recombinant Monoclonal Antibody [orb1733240]
IP, WB
Human, Mouse
Rabbit
Recombinant
Unconjugated
100 μl
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Detection of human NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 µg) from OVCAR-8, U-251MG, A-549, and UACC-257 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (orb1733239) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and C2C12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (orb1733239) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human NEDD-9/CASL/HEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (orb1733239) used for IP at 6 µl/mg lysate.
Quick Database Links
Gene Symbol
NEDD-9/CASL/HEF1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit anti-NEDD-9/CASL/HEF1 Recombinant Monoclonal Antibody (orb1733239)
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