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Rabbit RNF25 Antibody
SKU: orb1524077
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 409 and C-terminus |
| Target | RNF25 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−AO7; E3 ubiquitin-protein ligase RNF25; RING finger protein 25; ring finger protein AO7; RING-type E3 ubiquitin transferase RNF25
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Detection of human RNF25 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) , HEK293T (T; 50 µg) , and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody orb1524077 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human RNF25 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody orb1524077 used for IP at 6 µg/mg lysate.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_071898.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit RNF25 Antibody (orb1524077)
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