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Rabbit anti-RPA32 Antibody
SKU: orb1530480
Description
Images & Validation
−Item 1 of 5
| Tested Applications | FC, IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 to 1:10,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 220 and C-terminus |
| Target | RPA32 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−replication factor A protein 2; RPA32; RP-A p34; RP-A p32; RF-A protein 2; replication protein A 32 kDa subunit; REPA2; replication protein A 34 kDa subunit
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Detection of human RPA32 by western blot.Samples: Whole cell lysate from HEK293T, HeLa, Jurkat, and U2OS cells. Antibody: Affinity purified rabbit anti-RPA32 antibody at 0.1 µg/ml.
Detection of mouse RPA32 by western blot.Samples: Whole cell lysate from CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-RPA32 antibody at 0.04 µg/ml.
Detection of human RPA32 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RPA32 used at a dilution of 1:5, 000 (0.2 µg/ml).
Detection of mouse RPA32 by immunohistochemistry. Sample: FFPE section of mouse kidney. Antibody: Affinity purified rabbit anti-RPA32 used at a dilution of 1:5, 000 (0.2 µg/ml).
Detection of human RPA32 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-RPA32 antibody.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002937.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IP
Immunoprecipitation
Rabbit anti-RPA32 Antibody (orb1530480)
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