Smad1/5/9 rabbit pAb Antibody

SKU: orb766331

Featured

Description

Rabbit polyclonal antibody to Smad1/5/9

Images & Validation

−

Item 1 of 3

Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

−

Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human Smad1/5/9. AA range:10-59
Molecular Weight	56
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

−

Anti-SMAD1 antibody, anti-BSP1 antibody, anti-MADH1 antibody, anti-MADR1 antibody, anti-Mothers against decapentaplegic homolog 1 antibody, anti-MAD homolog 1 antibody, anti-Mothers against DPP homolog 1 antibody, anti-JV4-1 antibody, anti-Mad-related protein 1 antibody, anti-SMAD family member 1 antibody, anti-SMAD 1 antibody, anti-Smad1 antibody, anti-hSMAD1 antibody, anti-Transform antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western Blot analysis of various cells using Smad1/5/9 Polyclonal Antibody diluted at 1:500.

Western blot analysis of lysates from HeLa cells, using Smad1/5/9 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Immunofluorescence

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols