SMARCA4 Rabbit Polyclonal Antibody

SKU: orb10208

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Description

Research Area

Cancer Research, Cell Markers, Neuroscience, Nuclear Signaling Pathway, SWI/SNF (SWItch/Sucrose Non-Fermentable), Trithorax-group proteins (TrxG), Tumor Suppressors

Images & Validation

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Tested Applications	FC, IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/test
Reactivity	Human, Rat
Predicted Reactivity	Canine, Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human SMARCA4 (51-167/1647aa)
Target	SMARCA4
Molecular Weight	185 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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SMCA4_HUMAN; Transcription activator BRG1; EC:3.6.4.-; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A(BAF190A); Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; BAF190A; BRG1; SNF2B; SNF2L4; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; OTSC12; SNF2LB; hSNF2b; SNF2-beta;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Blank control: A549. Primary Antibody (green line): Rabbit Anti-BRG-1 antibody (orb10208), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 3 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BRG-1) Polyclonal Antibody, Unconjugated (orb10208) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BRG-1) Polyclonal Antibody, Unconjugated (orb10208) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

SMARCA4

UniProt

P51532

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence