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SPAM1 Antibody
SKU: orb1258275
Featured
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2). |
| Target | SPAM1 |
| Molecular Weight | Observed: 47kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−HEL-S-96n, HYA1, HYAL1, HYAL3, HYAL5, PH-2PH2SPAG15, hyaluronidase PH-2PH-20 hyaluronidase, epididymis secretory sperm binding protein Li 96n, hyal-PH2hyaluronoglucosaminidase PH-2sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding), sperm surface protein PH-20
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Western blot analysis of extracts of various cell lines, using SPAM1 antibody (orb1258275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using SPAM1 antibody (orb1258275). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
SPAM1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
SPAM1 Antibody (orb1258275)
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