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SPI1 Antibody (C-term)
SKU: orb1935429
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Porcine, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB34932 |
| Target | This SPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human SPI1. |
| Molecular Weight | 31083 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−Transcription factor PU1, 31 kDa-transforming protein, SPI1
Similar Products
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SPI1 Antibody (C-term) [orb2996976]
IF, IHC-P, WB
Mouse, Porcine, Rat
Human
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl
Quality Guarantee
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SPI1 Antibody (C-term) western blot analysis in WiDr cell line lysates (35 ug/lane). This demonstrates the SPI1 antibody detected the SPI1 protein (arrow).
SPI1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SPI1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody (C-term).NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). SPI1 immunoreactivity is localized to Nucleus significantly.
Quick Database Links
Gene Symbol
This SPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human SPI1.
UniProt
RefSeq (Protein):NP_001074016.1, NP_003111.2
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001074016.1, NP_003111.2 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
SPI1 Antibody (C-term) (orb1935429)
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