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SQSTM1 Antibody
SKU: orb1261282
Featured
Description
Research Area
Cell Biology, Signal Transduction
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 / p62 (NP_001135770.1). |
| Target | SQSTM1 |
| Molecular Weight | Observed: 62kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−SQSTM1 Antibody: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, ORCA, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP
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Western blot analysis of extracts of various cell lines, using SQSTM1 antibody (orb1261282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human tonsil using SQSTM1/p62 antibody (orb1261282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1/p62 antibody (orb1261282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using SQSTM1/p62 antibody (orb1261282) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1/p62 antibody (orb1261282) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of HeLa cells using SQSTM1/p62 antibody (orb1261282) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using SQSTM1/p62 antibody (orb1261282) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of C6 cells using 3 ug SQSTM1/p62 antibody (orb1261282). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (orb1261282) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
SQSTM1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
SQSTM1 Antibody (orb1261282)
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