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STAT5A Antibody

SKU: orb2636840

Description

Signal transducer and activator of transcription 5A (Stat5a) and Stat5b, which share 96% homology, undergo receptor tyrosine kinase or G protein-coupled receptor-dependent phosphorylation in response to cytokines or growth factors, and then form homo- or heterodimers that translocate to the nucleus, where they initiate transcription. Activation of Stat5a via IL-2, IL-3, IL-7/ GM-CSF, erythropoietin, thrombopoietin and growth hormones influences proliferation, differentiation and apoptosis in lymphohematopoietic cells. Phosphorylation of Stat5a at Ser127/Ser128 and Ser779 are contigent on ErbB-4- mediated activation of Stat5a. Activation of Stat5b via IL-2, IL-4, CSF-1 and growth hormones influences TCR signaling, apoptosis, adult mammary gland development and sexual dimorphism of liver gene expression. Stat5b is the major liver-expressed Stat5 form that has been shown to fuse with the retinoic acid receptor a gene in acute promyelocytic leukemias (APLL). Stat5a/b null mice have severely impaired lymphoid development and differentiation.

Images & Validation

Tested ApplicationsFACS
Dilution rangeFlow cytometry: 1-2ug/million cells
ReactivityHuman
Application Notes
Optimal dilution of the STAT5A antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.PCRP-STAT5A-1A11
ImmunogenSTAT5A protein was used as the immunogen for the STAT5A antibody.
PurificationProtein A/G affinity
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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STAT5A Antibody

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using STAT5A antibody (clone PCRP-STAT5A-1A11). These results demonstrate the foremost specificity of the PCRP-STAT5A-1A11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

STAT5A Antibody

FACS staining of PFA-fixed human HeLa cells with STAT5A antibody (blue, clone PCRP-STAT5A-1A11) and isotype control (red).

STAT5A Antibody

SDS-PAGE analysis of purified, BSA-free STAT5A antibody (PCRP-STAT5A-1A11) as confirmation of integrity and purity.

UniProt Details

No UniProt data available

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Protocol Information

FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol

STAT5A Antibody (orb2636840)

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Available Sizes

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100 μg
¥ 9,750.00
DispatchUsually dispatched within 5-10 working days
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