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Synuclein-α (phospho Tyr133) rabbit pAb Antibody

SKU: orb770123

Description

Rabbit polyclonal antibody to Synuclein-alpha (phospho-Tyr133)

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
ReactivityHuman, Mouse, Rat

Key Properties

ClonalityPolyclonal
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human Synuclein-alpha around the phosphorylation site of Tyr133. AA range:91-140
Molecular Weight18
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-SNCA antibody, anti-NACP antibody, anti-PARK1 antibody, anti-Alpha-synuclein antibody, anti-Non-A beta component of AD amyloid antibody, anti-Non-A4 component of amyloid precursor antibody, anti-NACP antibody
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Synuclein-α (phospho Tyr133) rabbit pAb Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Synuclein-alpha (Phospho-Tyr133) Antibody.

Synuclein-α (phospho Tyr133) rabbit pAb Antibody

Western blot analysis of lysates from 293 cells treated with Etoposide 25 muM 60', using Synuclein-alpha (Phospho-Tyr133) Antibody. The lane on the left is blocked with the phospho peptide.

Synuclein-α (phospho Tyr133) rabbit pAb Antibody

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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IF
Immunofluorescence
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Synuclein-α (phospho Tyr133) rabbit pAb Antibody (orb770123)

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50 μl
¥ 3,250.00
100 μl
¥ 5,070.00
DispatchUsually dispatched within 5-10 working days
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