TAGLN/Transgelin/SM22 Antibody

SKU: orb1537823

Description

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, IHC-P, WB
Dilution range	IHC, IHC-P (2.5 - 5 µg/ml), Peptide-ELISA (1:8000), WB (0.01 - 0.03 µg/ml)
Reactivity	Bovine, Canine, Equine, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rat, Xenopus
Application Notes	Further information: Immunohistochemistry: LS-B2563 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2563 was determined to be 5 ug/ml. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-MTGYGRPRQIIS, from the C-Terminus of protein sequence according to NP_003177.2.
Target	TAGLN / Transgelin / SM22
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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TAGLN, 22 kDa actin-binding protein, SMCC, TAGLN1, WS3-10, Transgelin, Transgelin variant 2, SM22, Protein WS3-10, SM22-alpha

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Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

Antibody staining (0.001 ug/ml) of Human Placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Quick Database Links

Gene Symbol

TAGLN / Transgelin / SM22

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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