TGFB4/LEFTY2 Antibody

SKU: orb1537819

Description

Images & Validation

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Tested Applications	ELISA, IHC, IHC-P, WB
Dilution range	ELISA (1:5000), IHC, IHC-P (10 µg/ml), WB (1:2000)
Reactivity	Human
Application Notes	Further information: Immunohistochemistry: LS-B2540 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2540 was determined to be 10 ug/ml. Read more...

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a,k
Clone No.	7C5G1H6H10
Immunogen	A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
Target	TGFB4 / LEFTY2
Purification	Protein A purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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LEFTY2, EBAF, LEFTYA, Protein lefty-2, LEFTA, TGFB4, Protein lefty-A, TGF-beta-4

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Quick Database Links

Gene Symbol

TGFB4 / LEFTY2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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