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VEGF-A rabbit pAb Antibody
SKU: orb770371
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Description
Images & Validation
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| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000.IF: 1:50-200 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Porcine, Rabbit, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human VEGF-A. AA range:110-159 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-VEGFA antibody, anti-VEGF antibody, anti-Vascular endothelial growth factor A antibody, anti-VEGF-A antibody, anti-Vascular permeability factor antibody, anti-VPF antibody
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Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
VEGF-A rabbit pAb Antibody (orb770371)
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