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Biotinylated Human Nectin-4
SKU: orb2992962
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Anti-Human Nectin-4 mAb(Cat# orb2993141) at 2μg/ml (100 μl/well) can bind Biotinylated Human Nectin-4 (C-6His-Avi)(Cat#orb2992962). The ED50 of Biotinylated Human Nectin-4 (C-6His-Avi)(Cat#orb2992962) is 0.035 ng/ml. (Regularly tested). BLI: Loaded Biotinylated Human Nectin-4 (C-6His-Avi) (Cat#orb2992962) on SA Biosensor, can bind Anti-Human Nectin-4 mAb (Cat#orb2993141) with an affinity constant of 3.74 pM as determined in BLI assay. (Regularly tested) |
| Tag | C-6His-Avi |
| Molecular Weight | Predicted: 16 KDa. Observed: 15-21 KDa, reducing conditions |
| Expression Region | Gly32-Leu153 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR
Similar Products
−Biotinylated Human Nectin-4 [orb2993794]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 37.2 KDa. Observed: 40-50 KDa, reducing conditions
20 μg, 100 μg
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Biotinylated Human Nectin-4 (orb2992962)
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