Biotinylated Human Nectin-4

SKU: orb2993794

Description

Biotinylated Recombinant Human Poliovirus Receptor-Related Protein 4 is produced by our Mammalian expression system and the target gene encoding Gly32-Val351 is expressed with a 6His, Avi tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Human Nectin-4-His-Avi(Cat#orb2993794) at 2μg/ml (100 μl/well) can bind Anti-Human Nectin-4 mAb-mFc(Cat#orb2993141). The ED50 of Anti-Human Nectin-4 mAb-mFc(Cat#orb2993141) is 12.28 ng/ml. (Regularly tested). BLI: Loaded Biotinylated Human Nectin-4-His-Avi(Cat#orb2993794) on SA Biosensor, can bind Anti-Human Nectin-4 mAb (Cat#orb2993141) with an affinity constant of 1 -3 nM as determined in BLI assay.
Tag	C-6His-Avi
Molecular Weight	Predicted: 37.2 KDa. Observed: 40-50 KDa, reducing conditions
Expression Region	Gly32-Val351
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Biotin

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR

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UniProt

Q96NY8

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No UniProt data available

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