CD44 Antibody / HCAM

SKU: orb749333

Description

This antibody recognizes a cell surface glycoprotein of 80-95kDa, called CD44, or HCAM, on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44-s). Higher molecular weight isoforms are described in epithelial cells (CD44-v), which are believed to function in intercellular adhesion and stromal binding. HCAM antibody immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution range	Flow cytometry: 2-4ug/million cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCAM antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a, kappa
Clone No.	156-3C11
Immunogen	Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Immunofluorescent staining of human HeLa cells with HCAM antibody (clone 156-3C11, green) and Reddot nuclear stain (red).

IHC testing of FFPE human tonsil stained with HCAM antibody (clone 156-3C11).

IHC testing of FFPE human breast cancer stained with HCAM antibody (clone 156-3C11).

Western blot testing of HeLa cell lysate with HCAM antibody (clone 156-3C11). Predicted molecular weight ~81 kDa.

SDS-PAGE analysis of purified, BSA-free HCAM antibody (clone 156-3C11) as confirmation of integrity and purity.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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