CD44 Antibody / HCAM

SKU: orb749903

Description

Recognizes a cell surface glycoprotein of 80-95kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. It is a receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. [UniProt]

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the CD44 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a, kappa
Clone No.	HCAM/918
Immunogen	Recombinant human protein was used as the immunogen for the CD44 antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Western blot testing of HeLa cell lysate with HCAM antibody (clone HCAM/918). Predicted molecular weight ~81 kDa.

Western blot of DU145 cell lysate using CD44 antibody (clone HCAM/918). Predicted molecular weight ~81 kDa.

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with CD44 antibody (clone HCAM/918).

Immunofluorescent staining of human HeLa cells with HCAM antibody (clone HCAM/918, green) and Reddot nuclear stain (red).

Quick Database Links

UniProt

P16070

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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