F(ab')2 Mouse IgG F(ab')2 Antibody Phycoerythrin Conjugated Pre-Adsorbed

SKU: orb348267

Description

F(ab')2 Mouse IgG F(ab')2 antibody (RPE)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, FC, IF, WB
Dilution Range	FC: 1:100 - 1:250, IF: 1:100 - 1:250, WB: 1:100 - 1:500
Reactivity	Mouse
Application Notes	F(ab')2 Anti-Mouse IgG F(ab')2 Phycoerythrin Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Read more...

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Mouse IgG F(ab')2 fragment
Purity	F(ab')2 fragment PE conjugated Anti-Mouse IgG F(ab')2 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse or Human Serum Proteins.
Conjugation	RPE

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Goat F(ab')2 Anti-Mouse IgG F(ab')2 Antibody phycoerythrin Conjugation, Goat Fab2 Anti-Mouse IgG Fab2 PE Conjugated Antibody

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F(ab')2 Mouse IgG F(ab')2 Antibody Phycoerythrin Conjugated Pre-Adsorbed

Western blot of Phycoerythrin conjugated Goat F(ab')2 Anti-Mouse IgG F(ab')2 Pre-Adsorbed secondary antibody. Lane 1: Mouse Fab2. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 kDa, 25 kDa for Mouse IgG F(ab')2. Other band(s): None.

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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DOT

Dot Blot

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