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F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin conjugated Pre-adsorbed
SKU: orb348268
Description
Images & Validation
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Item 1 of 1
| Tested Applications | DOT, FC, IF, WB |
|---|---|
| Dilution Range | FC: 1:100 - 1:250, IF: 1:100 - 1:250 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG F(ab')2 |
| Immunogen | F(ab')2 anti-Mouse IgG (H&L) was produced by repeated immunization with Mouse IgG whole molecule in goat. |
| Purity | F(ab')2 Anti-MOUSE IgG (H&L) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. F(ab')2 Anti-MOUSE IgG (H&L) (GOAT) Antibody assays by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine Chicken Goat Guinea Pig Horse Hamster Human Rabbit Rat and Sheep Serum Proteins. |
| Conjugation | RPE |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 0.5 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Goat F(ab')2 Anti-Mouse IgG Antibody Phycoerythrin Conjugation, Goat Fab2 Anti-Mouse IgG PE conjugated Antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
DOT
Dot Blot
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