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GART Mouse Monoclonal Antibody
SKU: orb1291534
Featured
Description
Research Area
Amino Acid Metabolism, Metabolism, Nucleotide Metabolism
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, WB |
|---|---|
| Dilution range | WB=1:500-1000, ICC/IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, Kappa |
| Clone No. | 1D14 |
| Immunogen | KLH conjugated synthetic peptide derived from human GART |
| Target | GART |
| Molecular Weight | 107 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS, Glycerol, rAlbumin. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−5'-phosphoribosylglycinamide transformylase; AIR synthase; AIRS; GAR transformylase; GARS; GARTF; Glycinamide ribonucleotide synthetase; MGC47764; PAIS; PGFT; phosphoribosyl-aminoimidazole synthetase; phosphoribosylglycinamide formyltransferase; phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase; phosphoribosylglycinamide formyltransferase, EC 2.1.2.29; phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase; phosphoribosylglycinamide synthetase; PRGS; PUR2_HUMAN; Trifunctional purine biosynthetic protein adenosine 3.
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Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: HepG2, 2: HEK-293, 3: PC-12, Protein loading quantity: 20 ug, Exposure time: 30 s, Predicted MW: 40, 108 kDa, Observed MW: 52, 120 kDa.
Cell line: HepG2, Fixation: 100% Ice-cold methanol, Permeabilization: 0.1% Triton X-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4°C, overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1291534.
Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human A431 cell lysates, Lane 3: Human HepG2 cell lysates, Primary: Anti-GART (orb1291534) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 107 kDa, Observed band size: 55, 130 kDa.
Quick Database Links
Gene Symbol
GART
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
GART Mouse Monoclonal Antibody (orb1291534)
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