GART Rabbit Monoclonal Antibody

SKU: orb548400

Description

Anti-GART Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Epigenetics & Chromatin, Metabolism Research, Molecular Biology, Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	ICC, IF, IHC, IP, WB
Dilution range	WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:20
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	FFD-7
Immunogen	A synthesized peptide derived from human GART
Target	Trifunctional purine biosynthetic protein adenosine-3 [Includes: Phosphoribosylamine--glycine ligase
Molecular Weight	63 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin.
Concentration	0.5mg/ml
Disclaimer	For research use only

Alternative Names

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Trifunctional purine biosynthetic protein adenosine-3; Phosphoribosylamine--glycine ligase; 6.3.4.13; Glycinamide ribonucleotide synthetase; GARS; Phosphoribosylglycinamide synthetase; Phosphoribosylformylglycinamidine cyclo-ligase; 6.3.3.1; AIR synthase; AIRS; Phosphoribosyl-aminoimidazole synthetase; Phosphoribosylglycinamide formyltransferase; 2.1.2.2; 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART; GART; PGFT, PRGS

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Western blot analysis of GART expression in HeLa cell lysate (orb548400). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GART monoclonal antibody (Catalog # orb548400) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for GART.

Quick Database Links

Gene Symbol

Trifunctional purine biosynthetic protein adenosine-3 [Includes: Phosphoribosylamine--glycine ligase

UniProt

P22102

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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Immunoprecipitation

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