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HSP22 Antibody
SKU: orb1822421
Description
Research Area
Cancer Biology, Cell Biology, Neuroscience, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IF, IHC, IP, WB |
|---|---|
| Dilution range | WB (1:1000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user. |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Human HSP22 |
| Target | HSP22 |
| Molecular Weight | 22kDa |
| Purification | Peptide Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−HSPB8, HSP22, Heat shock protein beta-8, Alpha-crystallin C chain, E2-induced gene 1 protein, Protein kinase H11, Small stress protein-like protein, Heat shock protein family B member 8, CRYAC, E2IG1, CMT2L, DHMN2, H11, Heat shock 22kDa protein 8, HMN2, HSB8
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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP22 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear Staining.
Western blot analysis of Rat Skeletal muscle lysates showing detection of HSP22 protein using Rabbit Anti-HSP22 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Rabbit Anti-HSP22 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Epidermis positive, dermal staining.
Immunohistochemistry analysis using Rabbit Anti-HSP22 Polyclonal Antibody. Tissue: Spinal cord. Species: Mouse. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. DAPI merged with Alexa 488.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_055180.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation
HSP22 Antibody (orb1822421)
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