Recombinant HSP60 Antibody

SKU: orb2636772

Description

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.

Images & Validation

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Tested Applications	IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human, Mouse, Rat
Application Notes	Optimal dilution of the recombinant HSP60 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG2a, kappa
Clone No.	rHSPD1/6495
Immunogen	Recombinant full-length human HSPD1 protein was used as the immunogen for the recombinant HSP60 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Western blot testing of human Hep2 cell lysate using recombinant HSP60 antibody (clone rHSPD1/6495). Predicted molecular weight: ~60 kDa.

IHC staining of FFPE human thyroid tissue with recombinant HSP60 antibody (clone rHSPD1/6495). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant HSP60 antibody (clone rHSPD1/6495) as confirmation of integrity and purity.

Quick Database Links

UniProt

P10809

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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