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HSP60 Antibody
SKU: orb1151535
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | Mouse IgG1, kappa |
| Clone No. | rHSPD1/6497 |
| Immunogen | Recombinant human full-length HSP60 protein was used as the immunogen for the recombinant HSP60 antibody. Its epitope is localized between amino acids 383-447 of human hsp60. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Western blot testing of human 1) Hep-2 and 2) HeLa cell lysate using recombinant HSP60 antibody (clone rHSPD1/6497). Predicted molecular weight: ~60 kDa.
Western blot testing of human Hep-2 cell lysate using recombinant HSP60 antibody (clone rHSPD1/6497). Predicted molecular weight: ~60 kDa.
IHC staining of FFPE human kidney tissue with recombinant HSP60 antibody (clone rHSPD1/6497). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant HSP60 antibody (clone rHSPD1/6497) as confirmation of integrity and purity.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
HSP60 Antibody (orb1151535)
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