Human IgG (H&L) Antibody Fluorescein Conjugated

SKU: orb347345

Description

Human IgG (H&L) antibody (FITC)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, FC, FLISA, IF
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Human
Application Notes	Anti-Human IgG (H&L) Fluorescein has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

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Antibody Type	Secondary Antibody
Host	Gallus
Clonality	Polyclonal
Isotype	IgG
Immunogen	Human IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken Serum, Human IgG and Human Serum.
Conjugation	FITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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chicken anti-Human IgG fluorescein conjugated Antibody, chicken anti-Human IgG Antibody FITC conjugation

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Human IgG (H&L) Antibody Fluorescein Conjugated

Dot Blot of Chicken Anti-HUMAN IgG Fluorescein Conjugated Antibody. Lane 1: 100 ng. Lane 2: 33.3 ng. Lane 3: 11.1 ng. Lane 4: 3.7 ng. Lane 5: 1.23 ng. Secondary Antibody: Ch-a-Hu IgG FITC Conj 1 µg/ml. Blocking Buffer: BlockOut orb348644 for 30 min at RT.

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Flow Cytometry

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DOT

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