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Human IgG (H&L) Antibody Fluorescein Conjugated
SKU: orb347261
Description
Images & Validation
−Item 1 of 1
| Tested Applications | DOT, FC, FLISA, IF |
|---|---|
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Human IgG whole molecule |
| Purity | This product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Mouse Serum, Human IgG and Human Serum. |
| Conjugation | FITC |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−mouse anti-Human IgG fluorescein conjugated Antibody, mouse anti-Human IgG Antibody FITC conjugation
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Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human IgG starting at 200 ng was spotted onto 0.45 µm nitrocellulose. After blocking in 5% Blotto (p/n orb348624) 1 Hour at 20°C, Anti-Human IgG (H&L) (MOUSE) Antibody Fluorescein Conjugated (p/n orb347261) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n orb348637).
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Human IgG (H&L) Antibody Fluorescein Conjugated (orb347261)
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