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Human IgG (H&L) Antibody Fluorescein Conjugated
SKU: orb346651
Description
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−Item 1 of 1
| Tested Applications | FC, FLISA, IF |
|---|---|
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Human IgG whole molecule |
| Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Mouse Serum, Human IgG and Human Serum. |
| Conjugation | FITC |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 10 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Mouse anti-Human IgG Fluorescein Conjugated Antibody, mouse anti-Human IgG FITC Conjugated Antibody, mouse anti-Human IgG Antibody Fluorescein Conjugation
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Biorbyt primary and DyLight conjugated secondary antibodies were used to detect: Human transferrin; Alpha 1 anti trypsin; and Human IgG in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in blocking buffer (orb348637) and incubated for 2 hrs at RT. Secondary antibodies diluted 1:10000 in blocking buffer and incubated ~ 1hr at 4°C.
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FC
Flow Cytometry
IF
Immunofluorescence
Human IgG (H&L) Antibody Fluorescein Conjugated (orb346651)
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