MEK1 N-Term Antibody

SKU: orb420325

Description

MEK1 antibody

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:40,000, WB: 1:10000
Reactivity	Human, Mouse, Rat
Application Notes	Anti-MEK 1 (RABBIT) antibody has been tested in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH.
Purity	This affinity purified antibody is directed against human MEK1 protein. Anti-MEK1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/ml
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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rabbit anti-MEK1 antibody, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MAP2K1, MEK, MEK 1, MKK1, PRKMK1, MEK-1, ERK activator kinase 1, MAPK/ERK kinase 1

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Western Blot of Anti-MEK1 N Term Antibody. Lane M: Molecular weight markers. Lane 1: MEK1 rec lysate. Lane 2: MEK2 rec lysate. Lane 3: Mouse Brain Whole cell Lysate (p/n orb348715). Load: 10 ng. Primary Antibody: Anti-MEK1 at 1 µg/ml overnight at 4°C. Secondary Antibody: Goat anti-Rabbit Peroxidase Conjugated Antibody (p/n orb347654) at 1:40000 for 30 min at RT. Blocking: BlockOut Universal Blocking buffer (p/n orb348644). Predicted MW: 43.5kDa.

Quick Database Links

UniProt

Q02750

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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