MIP-1 alpha Antibody

SKU: orb345216

Description

MIP-1 alpha antibody

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:10,000, WB: 1:1000
Reactivity	Mouse
Application Notes	This purified antibody has been tested in western blotting and is suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7-8 kDa in size corresponding to the mature 92 amino acid mouse MIP-1α/Ccl3 protein by western blotting in appropriate cell lysate or extract.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse MIP-1α protein produced in E.coli.
Purity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse MIP-1α protein. Cross-reactivity with MIP-1α from other sources has not been determined.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	None
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit anti-MIP-1α antibody, rabbit anti-MIP-1 alpha antibody, Mouse Macrophage Inflammatory Protein-1-alpha, Ccl3 cytokine, C-C motif chemokine 3, Small-inducible cytokine A3, Macrophage inflammatory protein 1-α, MIP-1-alpha, TY-5, SIS-alpha, Heparin-binding chemotaxis protein, L2G25B, Scya3 protein

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Anti-mouse MIP-1a in western blot shows detection of recombinant mouse MIP-1a/Ccl3 raised in E.coli. Recombinant truncated (0.1 µg, expect ~8kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Biorbyt's Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20000 in blocking buffer (p/n orb348637) for 30 min at RT.

Quick Database Links

UniProt

P10855

RefSeq (Protein):NP_035467.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_035467.1

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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