Rabbit anti-ARID1B Recombinant Monoclonal Antibody

SKU: orb1806438

Description

Images & Validation

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Item 1 of 9

Tested Applications	FC, ICC, IHC, IP, WB
Dilution range	WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Clone No.	BLR291L
Immunogen	Between 1425 and 1475
Target	ARID1B
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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CSS1; MRD12; Osa homolog 2; P250R; hOsa2; ELD/OSA1; ELD (eyelid)/OSA protein; DAN15; OSA2; BRG1-binding protein hELD/OSA1; BRG1-binding protein ELD/OSA1; BRG1-associated factor 250b; BAF250B; AT-rich interactive domain-containing protein 1B; AT rich interactive domain 1B (SWI1-like); ARID domain-containing protein 1B; 6A3-5; BRIGHT

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Rabbit anti-ARID1B Recombinant Monoclonal Antibody

Detection of mouse ARID1B by western blot. Samples: Whole cell lysate (25 µg) from RenCa, EL4, A20, J774A1, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human ARID1B by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, U2OS, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of human ARID1B by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1B/BAF250 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1B (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1B (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438) used for IP at 6 µl/mg lysate.

Detection of human ARID1B by immunocytochemistry. Sample: FFPE section of RKO cells. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1B by immunocytochemistry. Sample: FFPE section of ND7/23 cells. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806438). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

ARID1B

UniProt

Q8NFD5

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ICC

Immunocytochemistry

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Immunoprecipitation

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