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Rabbit ARID1B Recombinant Monoclonal Antibody
SKU: orb1806439
Description
Research Area
Cancer Biology, Neuroscience
Images & Validation
−Item 1 of 9
| Tested Applications | ICC, IHC, IP, WB |
|---|---|
| Dilution Range | Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : IP , ApplicationDilutionRange : 6 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000 (3-8% Tris-acetate gel recommended) |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR291L |
| Immunogen | Between 1425 and 1475 |
| Target | ARID1B |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−6A3-5;ARID domain-containing protein 1B;AT rich interactive domain 1B (SWI1-like);AT-rich interactive domain-containing protein 1B;BAF250B;BRG1-associated factor 250b;BRG1-binding protein ELD/OSA1;BRG1-binding protein hELD/OSA1;BRIGHT;CSS1;DAN15;ELD (eyelid)/OSA protein;ELD/OSA1;hOsa2;MRD12;Osa homolog 2;OSA2;P250R
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Detection of human ARID1B by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, U2OS, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Detection of mouse ARID1B by western blot. Samples: Whole cell lysate (25 µg) from RenCa, EL4, A20, J774A1, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse ARID1B/BAF250 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human ARID1B by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse ARID1B (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human ARID1B (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) used for IP at 6 µl/mg lysate.
Detection of mouse ARID1B by immunocytochemistry. Sample: FFPE section of ND7/23 cells. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human ARID1B by immunocytochemistry. Sample: FFPE section of RKO cells. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_059989.3 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit ARID1B Recombinant Monoclonal Antibody (orb1806439)
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