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Rabbit PA1 Antibody
SKU: orb1527448
Description
Images & Validation
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| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1 and 50 |
| Target | PA1 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−C16orf53; GAS; glutamate-rich coactivator associated with SRC1; glutamate-rich coactivator interacting with SRC1; glutamate-rich coactivator interacting with SRC1/NCOA1; PA1; PAXIP1-associated glutamate-rich protein 1; PAXIP1-associated protein 1; PTIP-associated 1 protein; PTIP-associated protein 1
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Detection of human PA1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PA1 antibody orb1527448 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. PA1 was also immunoprecipitated by rabbit anti-PA1 antibodies, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 1 minute (A) and 30 seconds (B).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_078792.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit PA1 Antibody (orb1527448)
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