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Rabbit Rubicon Antibody
SKU: orb1526396
Description
Images & Validation
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| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 922 and C-terminus |
| Target | Rubicon |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−baron; beclin-1 associated RUN domain containing protein; KIAA0226; RUBICON; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; run domain Beclin-1-interacting and cysteine-rich domain-containing protein; rundataxin; SCAR15
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Detection of human Rubicon by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rubicon antibody orb1526396 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Rubicon by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rubicon antibody orb1526396 used for IP at 6 µg per reaction.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_055502.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit Rubicon Antibody (orb1526396)
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