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Rubicon Antibody
SKU: orb55248
Description
Research Area
Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB (1:1000), ICC/IF (1:100); IHC-P (1:50) |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide from the N-terminal of Human Rubicon |
| Target | Rubicon |
| Molecular Weight | 95 kDa |
| Purification | Peptide Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Run domain Beclin-1-interacting and cysteine-rich domain-containing protein, KIAA0226, Rubicon, Baron, RUBCN, Beclin-1 associated RUN domain containing protein
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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.
Immunohistochemistry analysis using Rabbit Anti-Rubicon Polyclonal Antibody. Tissue: Tonsil. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~108 kDa Rubicon protein using Rabbit Anti-Rubicon Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~108 kDa.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001139114.1 |
|---|
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Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
Rubicon Antibody (orb55248)
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