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Rabbit TCP1 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 506 and C-terminus |
| Target | TCP1 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Disclaimer | For research use only |
Alternative Names
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Detection of human TCP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TCP1 antibody orb1524825 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. TCP1 was also immunoprecipitated by rabbit anti-TCP1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).

Detection of human TCP1 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-TCP1 used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Detection of human TCP1 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-TCP1 (Cat. No. orb1524825) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_110379.2 |
|---|
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Protocol Information
Rabbit TCP1 Antibody (orb1524825)
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Login to Submit a Review![TCP1 epsilon antibody [N1C1]](/images/pub/media/catalog/product/t/c/tcp1-epsilon-antibody_orb556674_ifa_1.jpg)
![TCP1 epsilon antibody [N1C1]](/images/pub/media/catalog/product/t/c/tcp1-epsilon-antibody_orb556674_ihc-p_1.jpg)
![TCP1 epsilon antibody [N1C1]](/images/pub/media/catalog/product/t/c/tcp1-epsilon-antibody_orb556674_ip_1.jpg)
![TCP1 epsilon antibody [N1C1]](/images/pub/media/catalog/product/t/c/tcp1-epsilon-antibody_orb556674_wb_1.jpg)
![TCP1 epsilon antibody [N1C1]](/images/pub/media/catalog/product/t/c/tcp1-epsilon-antibody_orb556674_wb_2.jpg)
![TCP1 theta antibody [N2C2], Internal](/images/pub/media/catalog/product/t/c/tcp1-theta-antibody_orb556277_ihc_1.jpg)
![TCP1 theta antibody [N2C2], Internal](/images/pub/media/catalog/product/t/c/tcp1-theta-antibody_orb556277_ihc-p_1.jpg)
![TCP1 theta antibody [N2C2], Internal](/images/pub/media/catalog/product/t/c/tcp1-theta-antibody_orb556277_wb_1.jpg)
![TCP1 theta antibody [N2C2], Internal](/images/pub/media/catalog/product/t/c/tcp1-theta-antibody_orb556277_wb_2.jpg)








