Rabbit CCT5 Antibody

SKU: orb1524757

Description

CCT5 Antibody

Research Area

Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

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Item 1 of 3

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 100 and 150
Target	CCT5
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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CCTE; CCT-epsilon; chaperonin containing TCP1, subunit 5 (epsilon); epididymis secretory protein Li 69; HEL-S-69; PNAS-102; T-complex protein 1 subunit epsilon; TCP-1-epsilon

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Detection of human and mouse CCT5 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , Jurkat (J; 50 µg) , HeLa (H; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CCT5 antibody orb1524757 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. CCT5 was also immunoprecipitated by rabbit anti-CCT5 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

Detection of mouse CCT5 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CCT5 (Cat. No. orb1524757) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human CCT5 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CCT5 (Cat. No. orb1524757) used at a dilution of 1: 200 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).

Quick Database Links

Gene Symbol

CCT5

UniProt

P48643

RefSeq (Protein):NP_036205.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_036205.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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