Rabbit TCP1 Antibody

SKU: orb1524827

Description

TCP1 Antibody

Research Area

Metabolism Research

Images & Validation

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Item 1 of 3

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 450 and 500
Target	TCP1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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CCT1; CCTa; CCT-alpha; D6S230E; tailless complex polypeptide 1; t-complex 1 protein; T-complex protein 1 subunit alpha; T-complex protein 1, alpha subunit; TCP-1-alpha

TCP1 beta Rabbit Polyclonal Antibody [orb156295]

Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Rat, Sheep, Zebrafish
Reactivity:	Human, Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

Item 1 of 3
Rabbit CCT5 Antibody [orb1524757]
Applications:
IHC, IP, WB
Predicted Reactivity:
Rat
Reactivity:
Human, Mouse
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl, 10 μl
Item 1 of 3
Rabbit CCT2 Antibody [orb1524761]
Applications:
IHC, IP, WB
Predicted Reactivity:
Bovine, Rat
Reactivity:
Human, Mouse
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl, 10 μl

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Detection of human and mouse TCP1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TCP1 antibody orb1524827 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. TCP1 was also immunoprecipitated by rabbit anti-TCP1 antibodies, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human TCP1 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-TCP1 used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Detection of human TCP1 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-TCP1 (Cat. No. orb1524827) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

TCP1

UniProt

P17987

RefSeq (Protein):NP_110379.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_110379.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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