Rabbit anti-Thrombomodulin Recombinant Monoclonal Antibody

SKU: orb1710832

Description

Thrombomodulin Antibody

Images & Validation

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Item 1 of 7

Tested Applications	FC, ICC, IHC, IP, WB
Dilution range	WB: 1:1,000, IP: 12 µl/1 mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Clone No.	BLR253L
Immunogen	Residues 17-515 (ECD)
Target	Thrombomodulin
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	500 µg/ml
Disclaimer	For research use only

Alternative Names

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CD141 antigen; AHUS6; TM; thrombomodulin; THRM; fetomodulin; CD141; CD antigen CD141; BDCA3; THPH12

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Rabbit anti-Thrombomodulin Recombinant Monoclonal Antibody

Detection of human Thrombomodulin by western blot. Samples: Whole cell lysate (50 µg) from A-549, MDA-MB-231, A-431 (25 µg) , THP-1, and CAL-27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human Thrombomodulin by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Thrombomodulin by immunohistochemistry. Sample: FFPE section of human lymph node. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Thrombomodulin (shaded) in THP-1 cells by flow cytometry. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human Thrombomodulin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-431 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832) used for IP at 12 µl/mg lysate.

Detection of human Thrombomodulin by immunocytochemistry. Sample: FFPE section of human SW954 cells. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Thrombomodulin by immunocytochemistry. Sample: FFPE section of human A-431 cells. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

Thrombomodulin

UniProt

P07204

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ICC

Immunocytochemistry

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Immunoprecipitation

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