Rabbit anti-Thrombomodulin Recombinant Monoclonal Antibody

SKU: orb1710821

Description

Thrombomodulin Antibody

Images & Validation

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Item 1 of 5

Tested Applications	FC, ICC, IHC, IP, WB
Dilution range	WB: 1:1,000, IP: 12 µl/1 mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Reactivity	Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Clone No.	BLR260L
Immunogen	Residues 17-517 (ECD)
Target	Thrombomodulin
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	500 µg/ml
Disclaimer	For research use only

Alternative Names

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CD141; TM; thrombomodulin; fetomodulin; CD antigen CD141; AI385582; snoRNA MBII-339

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Rabbit anti-Thrombomodulin Recombinant Monoclonal Antibody

Detection of mouse Thrombomodulin by western blot. Samples: Whole cell lysate (50 µg) from mIMCD-3, NIH 3T3, bEnd.3, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710821) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse Thrombomodulin by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710821). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse Thrombomodulin (shaded) in 7OZ/3 cells by flow cytometry. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710821) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse Thrombomodulin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from bEnd.3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710821) used for IP at 12 µl/mg lysate.

Detection of mouse Thrombomodulin by immunocytochemistry. Sample: FFPE section of mouse 7OZ/3 cells. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710821). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

Thrombomodulin

UniProt

P15306

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ICC

Immunocytochemistry

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Immunoprecipitation

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