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Raf-B rabbit pAb
SKU: orb766191
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human B-RAF. AA range:576-625 |
| Molecular Weight | 86 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-BRAF antibody, anti-BRAF1 antibody, anti-RAFB1 antibody, anti-Serine/threonine-protein kinase B-raf antibody, anti-Proto-oncogene B-Raf antibody, anti-p94 antibody, anti-v-Raf murine sarcoma viral oncogene homolog B1 antibody
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Western blot analysis in MCF-7 cells transfected with siB-Raf. Cancer Letters 355 (2014) 288–296.
Western Blot analysis of various cells using Raf-B Polyclonal Antibody diluted at 1:500.
Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, using B-RAF Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from K562 cells, using B-RAF Antibody. The lane on the right is blocked with the synthesized peptide.
Quick Database Links
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Raf-B rabbit pAb (orb766191)
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