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Raf-B (phospho Thr753) rabbit pAb
SKU: orb770153
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Description
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−Item 1 of 3
| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human B-Raf around the phosphorylation site of Thr753. AA range:717-766 |
| Molecular Weight | 84 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-BRAF antibody, anti-BRAF1 antibody, anti-RAFB1 antibody, anti-Serine/threonine-protein kinase B-raf antibody, anti-Proto-oncogene B-Raf antibody, anti-p94 antibody, anti-v-Raf murine sarcoma viral oncogene homolog B1 antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using B-Raf (Phospho-Thr753) Antibody.
Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30', using B-Raf (Phospho-Thr753) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Quick Database Links
Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Raf-B (phospho Thr753) rabbit pAb (orb770153)
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