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Ref-1 rabbit pAb Antibody
SKU: orb766212
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human APEX1. AA range:191-240 |
| Molecular Weight | 34 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-APEX1 antibody, anti-APE antibody, anti-APE1 antibody, anti-APEX antibody, anti-APX antibody, anti-HAP1 antibody, anti-REF1 antibody, anti-DNA-(apurinic or apyrimidinic site) lyase antibody, anti-APEX nuclease antibody, anti-APEN antibody, anti-Apurinic-apyrimidinic endonuclease 1 antibody, anti-AP endonuclease 1 antibody, anti-APE-1 antibody, anti-REF-1 antibody, anti-Redox factor-1 antibody
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Western Blot analysis of various cells using Ref-1 Polyclonal Antibody diluted at 1:2000.
Western Blot analysis of HeLa cells using Ref-1 Polyclonal Antibody diluted at 1:2000.
Western blot analysis of lysates from COLO205 and HeLa cells, using APEX1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Ref-1 rabbit pAb Antibody (orb766212)
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