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APEX1 Antibody (N-term)
SKU: orb1931397
Description
Research Area
Cardiovascular Research, Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB17544 |
| Target | This APEX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-64 amino acids from the N-terminal region of human APEX1. |
| Molecular Weight | 35555 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−DNA-(apurinic or apyrimidinic site) lyase, 31--, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial, APEX1, APE, APE1, APEX, APX, HAP1, REF1
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Western blot analysis of anti-APEX1 Antibody (N-term) in Hela cell line lysates (35 ug/lane). APEX1 (arrow) was detected using the purified Pab.
Western blot analysis of APEX1 (arrow) using rabbit polyclonal APEX1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APEX1 gene.
Confocal immunofluorescent analysis of APEX1 Antibody (N-term) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Quick Database Links
Gene Symbol
This APEX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-64 amino acids from the N-terminal region of human APEX1.
UniProt
RefSeq (Protein):NP_542380.1, NP_542379.1, NP_001632.2, NP_001231178.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_542380.1, NP_542379.1, NP_001632.2, NP_001231178.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
APEX1 Antibody (N-term) (orb1931397)
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