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VPS45 Antibody

SKU: orb1246787

Description

VPS45 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Testis and Peripheral Blood Mononucleocyte lysates (calculated MW of 65.1kDa according to NP_009190.2). Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex), Placenta and Small Intestine. Recommended concentration: 5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-ESSQVTSRSASRR
TargetVPS45
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

VPS45, VPS45A, VPS45B, VSP45, VSP45A, H1, H1VPS45, leucocyte vacuolar protein sorting 45, vacuolar protein sorting 45A, vacuolar protein sorting 45A (yeast homolog), vacuolar protein sorting 45 homolog (S. cerevisiae), vacuolar protein sorting 45A (yeast), vacuolar protein sorting 45B (yeast), vacuolar protein sorting protein similar to S. cerevisiae Vsp45p, RP11-458I7.2, VPS54A

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VPS45 Antibody

orb1246787 (0.3 ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

VPS45 Antibody

orb1246787 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

VPS45 Antibody

orb1246787 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

VPS45 Antibody

orb1246787 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

VPS45 Antibody (orb1246787)

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0.1 mg
¥ 11,960.00
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