VPS45 (C Terminus) Antibody

SKU: orb19289

Description

Goat polyclonal antibody to VPS45

Research Area

Epigenetics

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in Human Testis and Peripheral Blood Mononucleocyte lysates (calculated MW of 65.1kDa according to NP_009190.2). Recommended concentration: 0.3-1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	VPS45 (C Terminus)
Molecular Weight	65.1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti VPS45 antibody, anti VPS45A antibody, anti VPS45B antibody, anti VSP45 antibody, anti VSP45A antibody, anti H1 antibody, anti H1VPS45 antibody, anti leucocyte vacuolar protein sorting 45 antibody, anti vacuolar protein sorting 45A antibody, anti vacuolar protein sorting 45A (yeast homolog) antibody, anti vacuolar protein sorting 45 homolog (S. cerevisiae) antibody, anti vacuolar protein sorting 45A (yeast) antibody, anti vacuolar protein sorting 45B (yeast) antibody, anti vacuolar protein sorting protein similar to S. cerevisiae Vsp45p antibody, anti RP11-458I7.2 antibody, anti VPS54A antibody

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orb19289 (0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Cortex using VPS45 antibody

Immunohistochemical staining of Human Placenta using VPS45 antibody

Immunohistochemical staining of Human Small Intestine using VPS45 antibody

Quick Database Links

Gene Symbol

VPS45 (C Terminus)

NCBI Gene ID

11311

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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