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AKT1 Antibody
SKU: orb685573
Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | WB: 1: 500-1: 3000, IHC-P: 1: 50-1: 100, IF: 1: 100-1: 500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized non-phosphopeptide derived from Human Akt around the phosphorylation site of tyrosine 326 (N-D-Y(p)-G-R). |
| Target | AKT1 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Buffer/Preservatives | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Disclaimer | For research use only |
Alternative Names
−Anti-AKT antibody, anti-AKT1 kinase antibody, anti-C-AKT antibody, anti-EC 2.7.11.1 antibody, anti-PKB antibody
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Western blot analysis of HeLa cells and 293 cells using AKT1 (Ab-326) antibody
Immunohistochemical staining of human breast carcinoma tissue using AKT1 (Ab-326) antibody
Immunofluorescence analysis of HeLa cells using AKT1 (Ab-326) antibody
Western blot analysis of NIH/3T3 cells using AKT1 (Ab-326) antibody
Quick Database Links
Gene Symbol
AKT1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
AKT1 Antibody (orb685573)
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