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Description
Research Area
Metabolism Research
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:1000-1:2000, Immunofluorescence 1:200 – 1:500 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | [2H5] |
| Target | alpha 1-microglobulin |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate-buffered solutions, pH 7.2, 50% Glycerol, 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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anti-Alpha-1-microglobulin antibody [2H5] at 1/1000 dilution Lysates at 30 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1973111 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1973112 anti-Alpha-1-microglobulin antibody [2H5] (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Quick Database Links
Gene Symbol
alpha 1-microglobulin
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
alpha 1-microglobulin Mouse Monoclonal Antibody (orb1973111)
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