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Annexin IV Recombinant Mouse Monoclonal Antibody
SKU: orb1152027
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | D4C7 |
| Immunogen | KLH conjugated synthetic peptide derived from human Annexin IV |
| Target | ANXA4 |
| Molecular Weight | 35 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−35 beta calcimedin; Carbohydrate binding protein P33/P41; DKFZp686H02120; Endonexin I; Lipocortin IV; P32.5; Annexin A4; Annexin IV placental anticoagulant protein II; ANX 4; ANX A4; ANX4; ANXA 4; LipocortinIV; P33/41; Placental anticoagulant protein II antibody PP4X; Proliferation inducing protein 28; ANXA4_HUMAN.
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Lysate: HeLa, HepG2, SW480, SH-SY5Y, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 32 kDa, Observed MW: 32 kDa.
Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152027.
Tissue: Human placenta, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1152027.
Quick Database Links
Gene Symbol
ANXA4
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
Annexin IV Recombinant Mouse Monoclonal Antibody (orb1152027)
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