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CD277 Antibody
SKU: orb666153
Description
Research Area
Epigenetics, Immunology
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1-500-2000, IF: 1-50-200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human CD277. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BTF5; Butyrophilin subfamily 3 member A1; CD277
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Western blot analysis of CD277 expression in HL60 (A), U937 (B) whole cell lysates. (Predicted band size: 39; 41; 52; 57 kD; Observed band size: 56 kD)
Immunofluorescent analysis of CD277 staining in mouse lung tissues. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
CD277 Antibody (orb666153)
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